5Y00
Acid-tolerant monomeric GFP, Gamillus, fluorescence (ON) state
Summary for 5Y00
| Entry DOI | 10.2210/pdb5y00/pdb |
| Descriptor | Green fluorescent protein, PHOSPHATE ION, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | fluorescent protein, beta barrel, gfp-like protein, trans-chromophore |
| Biological source | Olindias |
| Total number of polymer chains | 1 |
| Total formula weight | 30630.74 |
| Authors | Nakashima, R.,Sakurai, K.,Shinoda, H.,Matsuda, T.,Nagai, T. (deposition date: 2017-07-14, release date: 2018-01-17, Last modification date: 2024-10-09) |
| Primary citation | Shinoda, H.,Ma, Y.,Nakashima, R.,Sakurai, K.,Matsuda, T.,Nagai, T. Acid-Tolerant Monomeric GFP from Olindias formosa. Cell Chem Biol, 25:330-338.e7, 2018 Cited by PubMed Abstract: The fluorescent protein (FP) color palette has greatly contributed to the visualization of molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than their neutral pK (∼6), and this has hampered their application in acidic organelles (pH ∼4.5-6.0). Currently, several cyan- and red-colored acid-tolerant FPs are available; however, there are few reports of acid-tolerant green FPs (GFPs) that are practically applicable to bioimaging. Here, we developed the acid-tolerant monomeric GFP "Gamillus" from the jellyfish Olindias formosa, with excellent brightness, maturation speed, and photostability. Results from X-ray crystallography and point mutagenesis suggest that across a broad pH range the acid tolerance is attributed to stabilization of deprotonation in the chromophore phenyl ring by forming a unique trans configuration. We demonstrate that Gamillus can serve as a molecular tag suitable for imaging in acidic organelles through autophagy-mediated molecular tracking to lysosomes. PubMed: 29290624DOI: 10.1016/j.chembiol.2017.12.005 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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