5Y00
Acid-tolerant monomeric GFP, Gamillus, fluorescence (ON) state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.900 |
Spacegroup name | I 21 3 |
Unit cell lengths | 161.158, 161.158, 161.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 113.960 - 1.600 |
R-factor | 0.14595 |
Rwork | 0.145 |
R-free | 0.15831 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.039 |
RMSD bond angle | 3.332 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 114.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.073 | 0.647 |
Number of reflections | 90910 | |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.9 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Ammonium phosphate, sodium citrate, sodium chloride, HEPES, pH5.5 |