5XU0

Structure of the membrane fusion protein Spr0693 from Streptococcus pneumoniae R6

Summary for 5XU0

• Entry DOI: 10.2210/pdb5xu0/pdb
• Descriptor: Membrane-fusion protein (1 entity in total)
• Functional Keywords: membrane fusion protein, channel, transport protein
• Biological source: Streptococcus pneumoniae
• Total number of polymer chains: 3
• Total formula weight: 87069.64

Authors

Yang, H.B.,Jiang, Y.L.,Hou, W.T.,Chen, M.T.,Chen, Y.,Zhou, C.Z. (deposition date: 2017-06-22, release date: 2018-01-24, Last modification date: 2024-10-30)

Primary citation

Yang, H.B.,Hou, W.T.,Cheng, M.T.,Jiang, Y.L.,Chen, Y.,Zhou, C.Z.
Structure of a MacAB-like efflux pump from Streptococcus pneumoniae.
Nat Commun, 9:196-196, 2018

PubMed Abstract: The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694-0695 at 3.3 Å and Spr0693 at 3.0 Å resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694-0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria.

PubMed: 29335499
DOI: 10.1038/s41467-017-02741-4

Experimental method

X-RAY DIFFRACTION (2.95 Å)