5XU0
Structure of the membrane fusion protein Spr0693 from Streptococcus pneumoniae R6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 159.210, 159.210, 99.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.981 - 2.950 |
| R-factor | 0.2504 |
| Rwork | 0.248 |
| R-free | 0.30520 |
| Structure solution method | SAD |
| Starting model (for MR) | 1f3o |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 2.950 | 3.000 |
| Number of reflections | 27463 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 285 | 7.5 % PEG 4000, 100 mM CaCl2, 10%-20% MPD |
