5XPD

Sugar transporter of AtSWEET13 in inward-facing state with a substrate analog

Summary for 5XPD

• Entry DOI: 10.2210/pdb5xpd/pdb
• Descriptor: sugar transporter, 2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE (2 entities in total)
• Functional Keywords: sweets sugar transporter complex, transport protein
• Biological source: Arabidopsis thaliana
• Total number of polymer chains: 1
• Total formula weight: 33313.86

Authors

Han, L.,Zhang, X.J. (deposition date: 2017-06-01, release date: 2017-09-13, Last modification date: 2023-11-22)

Primary citation

Han, L.,Zhu, Y.P.,Liu, M.,Zhou, Y.,Lu, G.Y.,Lan, L.,Wang, X.P.,Zhao, Y.F.,Zhang, X.C.
Molecular mechanism of substrate recognition and transport by the AtSWEET13 sugar transporter
Proc. Natl. Acad. Sci. U.S.A., 114:10089-10094, 2017

PubMed Abstract: Sugar Will Eventually be Exported Transporters (SWEETs) are recently identified sugar transporters that can discriminate and transport di- or monosaccharides across a membrane following the concentration gradient. SWEETs play key roles in plant biological processes, such as pollen nutrition, nectar secretion, seed filling, and phloem loading. SWEET13 from (AtSWEET13) is an important sucrose transporter in pollen development. Here, we report the 2.8-Å resolution crystal structure of AtSWEET13 in the inward-facing conformation with a substrate analog, 2'-deoxycytidine 5'-monophosphate, bound in the central cavity. In addition, based on the results of an in-cell transport activity assay and single-molecule Förster resonance energy transfer analysis, we suggest a mechanism for substrate selectivity based on the size of the substrate-binding pocket. Furthermore, AtSWEET13 appears to form a higher order structure presumably related to its function.

PubMed: 28878024
DOI: 10.1073/pnas.1709241114

Experimental method

X-RAY DIFFRACTION (2.788 Å)