5XOW

Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 6'A7' on the target strand

Summary for 5XOW

• Entry DOI: 10.2210/pdb5xow/pdb
• Descriptor: TtAgo (D546N), DNA (5'-D(P*(TD)P*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3'), RNA (5'-R(P*UP*AP*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*AP*CP*CP*UP*CP*G)-3'), ... (4 entities in total)
• Functional Keywords: argonaute, bulge, mirna, mismatch, dna binding protein
• Biological source: Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039); More
• Total number of polymer chains: 3
• Total formula weight: 89612.11

Authors

Sheng, G.,Wang, J.,Zhao, H.,Wang, Y. (deposition date: 2017-05-31, release date: 2017-10-04, Last modification date: 2023-11-22)

• Primary citation: Sheng, G.,Gogakos, T.,Wang, J.,Zhao, H.,Serganov, A.,Juranek, S.,Tuschl, T.,Patel, D.J.,Wang, Y.; Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes; Nucleic Acids Res., 45:9149-9163, 2017; PubMed: 28911094; DOI: 10.1093/nar/gkx547

Experimental method

X-RAY DIFFRACTION (2.902 Å)