5XON

RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS

Summary for 5XON

• Entry DOI: 10.2210/pdb5xon/pdb
• EMDB information: 6747
• Descriptor: DNA-directed RNA polymerase subunit, RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III, RNA polymerase II subunit B12.5, ... (20 entities in total)
• Functional Keywords: transcription, complex, transcription-rna complex, transcription/rna
• Biological source: Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast); More
• Total number of polymer chains: 18
• Total formula weight: 652138.46

Authors

Ehara, H.,Yokoyama, T.,Shigematsu, H.,Shirouzu, M.,Sekine, S. (deposition date: 2017-05-29, release date: 2017-08-16, Last modification date: 2024-03-27)

Primary citation

Ehara, H.,Yokoyama, T.,Shigematsu, H.,Yokoyama, S.,Shirouzu, M.,Sekine, S.I.
Structure of the complete elongation complex of RNA polymerase II with basal factors
Science, 357:921-924, 2017

PubMed Abstract: In the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex for processive transcription. Here we report the structure of the Pol II elongation complex bound with the basal elongation factors Spt4/5, Elf1, and TFIIS. Spt4/5 (the Spt4/Spt5 complex) and Elf1 modify a wide area of the Pol II surface. Elf1 bridges the Pol II central cleft, completing a "DNA entry tunnel" for downstream DNA. Spt4 and the Spt5 NGN and KOW1 domains encircle the upstream DNA, constituting a "DNA exit tunnel." The Spt5 KOW4 and KOW5 domains augment the "RNA exit tunnel," directing the exiting nascent RNA. Thus, the elongation complex establishes a completely different transcription and regulation platform from that of the initiation complexes.

PubMed: 28775211
DOI: 10.1126/science.aan8552

Experimental method

ELECTRON MICROSCOPY (3.83 Å)