5XON
RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit | polymer | 1743 | 194107.4 | 1 | UniProt (C4R4Y0) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb1 |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1227 | 139746.1 | 1 | UniProt (C4QZQ7) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb2 |
| 3 | C (C) | RNA polymerase II third largest subunit B44, part of central core | polymer | 304 | 34216.3 | 1 | UniProt (C4R7L2) Pfam (PF01193) Pfam (PF01000) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb3 |
| 4 | D (D) | RNA polymerase II subunit B32 | polymer | 186 | 20623.0 | 1 | UniProt (C4R2U9) Pfam (PF03874) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb4 |
| 5 | E (E) | RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III | polymer | 214 | 24962.7 | 1 | UniProt (C4R3P8) Pfam (PF03871) Pfam (PF01191) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb5 |
| 6 | F (F) | RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III | polymer | 155 | 17803.6 | 1 | UniProt (C4R1V1) Pfam (PF01192) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb6 |
| 7 | G (G) | RNA polymerase II subunit | polymer | 171 | 18802.6 | 1 | UniProt (C4R9A1) Pfam (PF03876) Pfam (PF00575) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb7 |
| 8 | H (H) | RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III | polymer | 145 | 16249.2 | 1 | UniProt (C4R273) Pfam (PF03870) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb8 |
| 9 | I (I) | DNA-directed RNA polymerase subunit | polymer | 115 | 13612.3 | 1 | UniProt (C4QY79) Pfam (PF02150) Pfam (PF01096) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb9 |
| 10 | J (J) | RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III | polymer | 72 | 8554.1 | 1 | UniProt (C4R009) Pfam (PF01194) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb10 |
| 11 | K (K) | RNA polymerase II subunit B12.5 | polymer | 118 | 13832.9 | 1 | UniProt (C4R3Z5) Pfam (PF13656) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb11 |
| 12 | L (L) | RNA polymerase subunit, found in RNA polymerase complexes I, II, and III | polymer | 72 | 7862.0 | 1 | UniProt (C4QWA8) Pfam (PF03604) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | RNA polymerase II subunit Rpb12 |
| 13 | M (P) | RNA (30-MER) | polymer | 30 | 9492.6 | 1 | synthetic construct | ||
| 14 | N (T) | DNA (48-MER) | polymer | 48 | 14659.4 | 1 | synthetic construct | ||
| 15 | O (N) | DNA (48-MER) | polymer | 48 | 14917.5 | 1 | synthetic construct | ||
| 16 | P (U) | General transcription elongation factor TFIIS | polymer | 190 | 21694.7 | 1 | UniProt (C4R2R6) Pfam (PF07500) Pfam (PF01096) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | |
| 17 | Q (V) | Transcription elongation factor SPT4 | polymer | 108 | 12039.6 | 1 | UniProt (C4R0E6) Pfam (PF06093) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | |
| 18 | R (W) | Protein that forms a complex with Spt4p | polymer | 612 | 68284.0 | 1 | UniProt (C4R370) Pfam (PF03439) Pfam (PF00467) | Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) | Spt5 |
| 19 | AA, BA, CA, S, T... (L, U, V, A, B...) | ZINC ION | non-polymer | 65.4 | 10 | Chemie (ZN) | |||
| 20 | U (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
I: 3 - 115 (UniProt: C4QY79)
L: 1 - 72 (UniProt: C4QWA8)
U: 99 - 285 (UniProt: C4R2R6)
V: 8 - 114 (UniProt: C4R0E6)
W: 206 - 815 (UniProt: C4R370)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | see sequence details |
| Ala 2 | - | see sequence details |
| Ser 3 | Met 1 | see sequence details |
| Phe 4 | Thr 2 | see sequence details |
| Arg 5 | Asn 3 | see sequence details |
| Phe 6 | Val 4 | see sequence details |
| Cys 7 | Asn 5 | see sequence details |
| Leu 8 | Ser 6 | see sequence details |
| Glu 9 | Leu 7 | see sequence details |
| Cys 10 | Ser 8 | see sequence details |
| PDB | External Database | Details |
|---|---|---|
| Ile 67 | Ser 67 | see sequence details |
| Gln 68 | Lys 68 | see sequence details |
| Asp 70 | Leu 70 | see sequence details |
| Ala 71 | Thr 71 | see sequence details |
| Arg 72 | Thr 72 | see sequence details |
| PDB | External Database | Details |
|---|---|---|
| Gly 96 | - | expression tag |
| Pro 97 | - | expression tag |
| Gly 98 | - | expression tag |
| Ala 266 | Asp 266 | conflict |
| Ala 267 | Glu 267 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 7 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 204 | - | expression tag |
| Pro 205 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 651460.1 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 678.4 | |
| All* | Total formula weight | 652138.5 |
