5XON
RNA Polymerase II elongation complex bound with Spt4/5 and TFIIS
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.83 |
| Software | Gautomatch,RELION,SerialEM,PHENIX,Gctf,Chimera,Coot |
| Symmetry | C1 |
| CTF correction | NONE |
