Crystal structure of the branched-chain polyamine synthase (BpsA) in complex with N4-aminopropylspermidine and 5-methylthioadenosine

Summary for 5XNC

DescriptorN(4)-bis(aminopropyl)spermidine synthase, 5'-DEOXY-5'-METHYLTHIOADENOSINE, N,N-bis(3-aminopropyl)butane-1,4-diamine, ... (8 entities in total)
Functional Keywordstransferase, polyamine biosynthesis, spermidine, n4-aminopropylspermidine, branched polyamines
Biological sourceThermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
Total number of polymer chains7
Total molecular weight301701.6
Mizohata, E.,Tse, K.M.,Fujita, J.,Inoue, T. (deposition date: 2017-05-22, release date: 2018-08-15)
Primary citation
Hidese, R.,Tse, K.M.,Kimura, S.,Mizohata, E.,Fujita, J.,Horai, Y.,Umezawa, N.,Higuchi, T.,Niitsu, M.,Oshima, T.,Imanaka, T.,Inoue, T.,Fujiwara, S.
Active site geometry of a novel aminopropyltransferase for biosynthesis of hyperthermophile-specific branched-chain polyamine.
FEBS J., 284:3684-3701, 2017
PubMed: 28881427 (PDB entries with the same primary citation)
DOI: 10.1111/febs.14262
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.21330.0%4.6%6.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report