5XH3

Crystal structure of a novel PET hydrolase R103G/S131A mutant in complex with HEMT from Ideonella sakaiensis 201-F6

Summary for 5XH3

• Entry DOI: 10.2210/pdb5xh3/pdb
• Related: 5XH2
• Descriptor: Poly(ethylene terephthalate) hydrolase, O 4-(2-hydroxyethyl) O 1-methyl benzene-1,4-dicarboxylate, SULFATE ION, ... (5 entities in total)
• Functional Keywords: poly(ethylene terephthalate) hydrolase, substrate binding, inhibitor, hydrolase
• Biological source: Ideonella sakaiensis (strain 201-F6)
• Cellular location: Secreted : A0A0K8P6T7
• Total number of polymer chains: 1
• Total formula weight: 27743.64

Authors

Han, X.,Liu, W.D.,Zheng, Y.Y.,Chen, C.C.,Guo, R.T. (deposition date: 2017-04-19, release date: 2017-12-20, Last modification date: 2024-11-20)

Primary citation

Han, X.,Liu, W.,Huang, J.W.,Ma, J.,Zheng, Y.,Ko, T.P.,Xu, L.,Cheng, Y.S.,Chen, C.C.,Guo, R.T.
Structural insight into catalytic mechanism of PET hydrolase
Nat Commun, 8:2106-2106, 2017

PubMed Abstract: PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.

PubMed: 29235460
DOI: 10.1038/s41467-017-02255-z

Experimental method

X-RAY DIFFRACTION (1.3 Å)