5XH3
Crystal structure of a novel PET hydrolase R103G/S131A mutant in complex with HEMT from Ideonella sakaiensis 201-F6
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Poly(ethylene terephthalate) hydrolase | polymer | 261 | 27235.2 | 1 | UniProt (A0A0K8P6T7) Pfam (PF12740) | Ideonella sakaiensis (strain 201-F6) | PETase |
| 2 | B (A) | O 4-(2-hydroxyethyl) O 1-methyl benzene-1,4-dicarboxylate | non-polymer | 224.2 | 1 | Chemie (856) | |||
| 3 | C, D (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | E (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | F (A) | water | water | 18.0 | 244 | Chemie (HOH) |
Sequence modifications
A: 1 - 261 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Gly 103 | Arg 132 | engineered mutation |
| Ala 131 | Ser 160 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27235.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 508.4 | |
| All* | Total formula weight | 27743.6 |
