5XE1

Crystal structure of the indoleamine 2,3-dioxygenagse 1 (IDO1) complexed with INCB14943

Summary for 5XE1

• Entry DOI: 10.2210/pdb5xe1/pdb
• Descriptor: Indoleamine 2,3-dioxygenase 1, PROTOPORPHYRIN IX CONTAINING FE, 4-Amino-N-(3-chloro-4-fluorophenyl)-N'-hydroxy-1,2,5-oxadiazole-3-carboxamidine (3 entities in total)
• Functional Keywords: ido1, incb14943, oxidoreductase
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm, cytosol : P14902
• Total number of polymer chains: 2
• Total formula weight: 92544.67

Authors

Xu, J.,Wu, U.,Liu, J. (deposition date: 2017-03-31, release date: 2017-05-03, Last modification date: 2024-10-23)

Primary citation

Wu, Y.,Xu, T.,Liu, J.,Ding, K.,Xu, J.
Structural insights into the binding mechanism of IDO1 with hydroxylamidine based inhibitor INCB14943
Biochem. Biophys. Res. Commun., 487:339-343, 2017

PubMed Abstract: IDO1 (indoleamine 2, 3-dioxygenase 1), a well characterized immunosuppressive enzyme, has attracted growing attention as a potential target for cancer immunotherapy. Hydroxylamidine compounds INCB024360 and INCB14943 (INCB024360 analogue) are highly effective IDO1 inhibitors. INCB024360 is undergoing clinical trials for treatment of various types of human cancer. Here, we determined the co-crystal structure of IDO1 and INCB14943, and elucidate the detailed binding mode. INCB14943 binds to heme iron in IDO1 protein through the oxime nitrogen. Further analysis also reveals that a halogen bonding interaction between the chlorine atom (3-Cl) of INCB14943 and the sulphur atom of C129 significantly improves the inhibition activity against IDO1. Comparing with the other reported inhibitors, the oxime nitrogen and halogen bond interaction are identified as the unique features of INCB14943 among the IDO1 inhibitors. Thus, our study provides novel insights into the interaction between a small molecule inhibitor INCB14943 and IDO1 protein. The structural information will facilitate future IDO1 inhibitor design.

PubMed: 28412361
DOI: 10.1016/j.bbrc.2017.04.061

Experimental method

X-RAY DIFFRACTION (3.2 Å)