Crystal structure of Thermus thermophilus transcription initiation complex with GpA and pseudouridimycin (PUM)

Summary for 5X21

DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (12 entities in total)
Functional Keywordstranscription, rna polymerase, transcription inhibition, nucleoside-analog inhibitor, pseudouridimycin, transferase-dna complex, transferase/dna
Biological sourceThermus thermophilus
Cellular locationCytoplasm  Q72L95
Total number of polymer chains9
Total molecular weight445097.09
Zhang, Y.,Ebright, R. (deposition date: 2017-01-29, release date: 2017-07-05, Last modification date: 2018-01-17)
Primary citation
Maffioli, S.I.,Zhang, Y.,Degen, D.,Carzaniga, T.,Del Gatto, G.,Serina, S.,Monciardini, P.,Mazzetti, C.,Guglierame, P.,Candiani, G.,Chiriac, A.I.,Facchetti, G.,Kaltofen, P.,Sahl, H.G.,Deho, G.,Donadio, S.,Ebright, R.H.
Antibacterial Nucleoside-Analog Inhibitor of Bacterial RNA Polymerase.
Cell, 169:1240-1248.e23, 2017
PubMed: 28622509 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2017.05.042
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.28150.1%2.2%1.2%0.00MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5x21
no rotation
Molmil generated image of 5x21
rotated about x axis by 90°
Molmil generated image of 5x21
rotated about y axis by 90°