5X21
Crystal structure of Thermus thermophilus transcription initiation complex with GpA and pseudouridimycin (PUM)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 2 | UniProt (Q9Z9H6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (Q5SHR6) | Thermus thermophilus | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 1 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1524 | 170997.4 | 1 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11533.3 | 1 | UniProt (Q72ID6) Pfam (PF01192) UniProt (by SIFTS) (Q8RQE7) | Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor SigA | polymer | 443 | 50739.4 | 1 | UniProt (Q72L95) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (Q5SKW1) | Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) | |
| 6 | G (G) | promoter DNA template strand | polymer | 21 | 6464.2 | 1 | Thermus thermophilus | ||
| 7 | H (H) | promoter DNA nontemplate strand | polymer | 27 | 8421.4 | 1 | Thermus thermophilus | ||
| 8 | I (I) | RNA (5'-R(*GP*A)-3') | polymer | 2 | 629.5 | 1 | Thermus thermophilus | ||
| 9 | J, K, L, O, P... (A, C, D, F, H) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 10 | M, N (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 11 | R (I) | (1S)-1,4-anhydro-5-[(N-carbamimidoylglycyl-N~2~-hydroxy-L-glutaminyl)amino]-5-deoxy-1-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-D-ribitol | non-polymer | 486.4 | 1 | Chemie (PUM) | |||
| 12 | S, T, U, V, W... (A, B, C, D, E...) | water | water | 18.0 | 35 | Chemie (HOH) |
Sequence modifications
F: 1 - 423 (UniProt: Q72L95)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 444334.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 763.1 | |
| All* | Total formula weight | 445097.1 |
