5WX9

Crystal Structure of AtERF96 with GCC-box

Summary for 5WX9

• Entry DOI: 10.2210/pdb5wx9/pdb
• Descriptor: Ethylene-responsive transcription factor ERF096, GCC-box motif, ... (4 entities in total)
• Functional Keywords: protein-dna complex, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Arabidopsis thaliana (Mouse-ear cress); More
• Total number of polymer chains: 3
• Total formula weight: 21074.15

Authors

Chen, C.Y.,Cheng, Y.S. (deposition date: 2017-01-06, release date: 2017-11-22, Last modification date: 2023-11-22)

Primary citation

Chen, C.Y.,Lin, P.H.,Chen, K.H.,Cheng, Y.S.
Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box.
Plant Mol.Biol., 104:483-498, 2020

PubMed Abstract: The phytohormone ethylene is widely involved in many developmental processes and is a crucial regulator of defense responses against biotic and abiotic stresses in plants. Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis-acting element of target DNA. A previous study showed only the NMR structure of the AP2/ERF domain of AtERF100 in complex with a GCC box DNA motif. In this report, we determined the crystal structure of AtERF96 in complex with a GCC box at atomic resolution. We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. In addition to the AP2/ERF domain, an N-terminal α-helix of AtERF96 participates in DNA interaction in the flanking region. We also demonstrated the structure of AtERF96 EDLL motif, a unique conserved motif in the group IX of AP2/ERF family, might involve in the transactivation of defense-related genes. Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain.

PubMed: 32813232
DOI: 10.1007/s11103-020-01052-5

Experimental method

X-RAY DIFFRACTION (1.76 Å)