5WX9
Crystal Structure of AtERF96 with GCC-box
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97622 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.976, 81.181, 38.999 |
Unit cell angles | 90.00, 120.04, 90.00 |
Refinement procedure
Resolution | 21.120 - 1.760 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gcc |
RMSD bond length | 0.014 |
RMSD bond angle | 1.773 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.051 | 0.341 |
Number of reflections | 20152 | |
<I/σ(I)> | 14.3 | |
Completeness [%] | 97.3 | 98.9 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 297 | 0.02M Magnesium chloride hexahydrate &, 0.05M MOPS pH 7.0 &, 55% Tacsimate pH 7.0 |