Ligand-free US28 with stabilizing intracellular nanobody

Summary for 5WB1

DescriptorEnvelope protein US28, nanobody 7 fusion protein (1 entity in total)
Functional Keywordschemokine receptor, membrane protein
Biological sourceHuman cytomegalovirus (HHV-5)
Total number of polymer chains1
Total molecular weight51827.99
Jude, K.M.,Burg, J.S.,Garcia, K.C. (deposition date: 2017-06-27, release date: 2018-06-13)
Primary citation
Miles, T.F.,Spiess, K.,Jude, K.M.,Tsutsumi, N.,Burg, J.S.,Ingtram, J.R.,Hjorto, G.M.,Waghray, D.,Ploegh, H.L.,Rosenkilde, M.M.,Garcia, K.C.
Tuning GPCR signaling bias with engineered chemokine ligands
To Be Published,
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.28950.2%1.1%1.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5wb1
no rotation
Molmil generated image of 5wb1
rotated about x axis by 90°
Molmil generated image of 5wb1
rotated about y axis by 90°
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