5WB1
Ligand-free US28 with stabilizing intracellular nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.033200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.600, 38.300, 99.800 |
| Unit cell angles | 90.00, 107.40, 90.00 |
Refinement procedure
| Resolution | 29.844 - 3.508 |
| R-factor | 0.2471 |
| Rwork | 0.242 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xt1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | XDS (March 1, 2015) |
| Data scaling software | XSCALE (March 1, 2015) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.580 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.022 | 1.392 |
| Rmeas | 1.583 | |
| Rpim | 0.024 | |
| Number of reflections | 7426 | 491 |
| <I/σ(I)> | 6.85 | 0.94 |
| Completeness [%] | 99.2 | 91.8 |
| Redundancy | 6.3 | 4.2 |
| CC(1/2) | 0.994 | 0.606 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 37-41% PEG300, 100 mM MES, pH 6.2-6.6, 100 mM ammonium tartrate in monoolein:cholesterol (9:1) |
