5W52

MicroED structure of the segment, DLIIKGISVHI, from the RRM2 of TDP-43, residues 247-257

Summary for 5W52

• Entry DOI: 10.2210/pdb5w52/pdb
• Related: 5W50
• EMDB information: 8765
• Descriptor: TAR DNA-binding protein 43 (1 entity in total)
• Functional Keywords: amyloid, steric zipper, protein fibril
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 1209.48

Authors

Guenther, E.L.,Sawaya, M.R.,Cascio, D.,Eisenberg, D.S. (deposition date: 2017-06-13, release date: 2018-02-21, Last modification date: 2024-04-03)

Primary citation

Guenther, E.L.,Ge, P.,Trinh, H.,Sawaya, M.R.,Cascio, D.,Boyer, D.R.,Gonen, T.,Zhou, Z.H.,Eisenberg, D.S.
Atomic-level evidence for packing and positional amyloid polymorphism by segment from TDP-43 RRM2.
Nat. Struct. Mol. Biol., 25:311-319, 2018

PubMed Abstract: Proteins in the fibrous amyloid state are a major hallmark of neurodegenerative disease. Understanding the multiple conformations, or polymorphs, of amyloid proteins at the molecular level is a challenge of amyloid research. Here, we detail the wide range of polymorphs formed by a segment of human TAR DNA-binding protein 43 (TDP-43) as a model for the polymorphic capabilities of pathological amyloid aggregation. Using X-ray diffraction, microelectron diffraction (MicroED) and single-particle cryo-EM, we show that the DLIIKGISVHI segment from the second RNA-recognition motif (RRM2) forms an array of amyloid polymorphs. These associations include seven distinct interfaces displaying five different symmetry classes of steric zippers. Additionally, we find that this segment can adopt three different backbone conformations that contribute to its polymorphic capabilities. The polymorphic nature of this segment illustrates at the molecular level how amyloid proteins can form diverse fibril structures.

PubMed: 29531287
DOI: 10.1038/s41594-018-0045-5

Experimental method

ELECTRON CRYSTALLOGRAPHY (1.4 Å)