5W32
Crystal structure of mutant CJ YCEI protein (CJ-N48C) with selenocysteine guest structure
Summary for 5W32
| Entry DOI | 10.2210/pdb5w32/pdb |
| Related | 5W17 5W2X |
| Descriptor | Putative periplasmic protein, UNKNOWN LIGAND, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | nanotechnology nanoporous, unknown function |
| Biological source | Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) |
| Total number of polymer chains | 1 |
| Total formula weight | 20831.56 |
| Authors | Huber, T.R.,Snow, C.D. (deposition date: 2017-06-07, release date: 2018-01-03, Last modification date: 2023-10-04) |
| Primary citation | Huber, T.R.,McPherson, E.C.,Keating, C.E.,Snow, C.D. Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals. Bioconjug. Chem., 29:17-22, 2018 Cited by PubMed Abstract: Protein crystals are porous self-assembling materials that can be rapidly evolved by mutagenesis. We aimed to develop scaffold assisted crystallography techniques in an engineered protein crystal with large pores (>13 nm). Guest molecules were installed via a single covalent bond to attempt to reduce the conformational freedom and achieve high-occupancy structures. We used four different conjugation strategies to attach guest molecules to three different cysteine sites within pre-existing protein crystals. In all but one case, the presence of the adduct was obvious in the electron density. Structure determination of larger guest molecules may be feasible due to the large pores of the engineered scaffold crystals. PubMed: 29232505DOI: 10.1021/acs.bioconjchem.7b00668 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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