5VW1

Crystal structure of SpyCas9-sgRNA-AcrIIA4 ternary complex

Summary for 5VW1

Entry DOI	10.2210/pdb5vw1/pdb
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, sgRNA, anti-CRISPR protein AcrIIA4, ... (8 entities in total)
Functional Keywords	type ii crispr-cas endonculease: cas9: structure: endonuclease: anti-crispr protein: inhibition of cas9: ruvc catalytic pocket: sequence-specific pam recognition: genome editing tool, hydrolase
Biological source	Streptococcus pyogenes serotype M1 More
Total number of polymer chains	3
Total formula weight	197146.49
Authors	Yang, H.,Patel, D.J. (deposition date: 2017-05-21, release date: 2017-06-28, Last modification date: 2023-10-04)
Primary citation	Yang, H.,Patel, D.J. Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9. Mol. Cell, 67:117-127.e5, 2017 Cited by PubMed: 28602637 DOI: 10.1016/j.molcel.2017.05.024 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.598 Å)

Structure validation