5VW1

Crystal structure of SpyCas9-sgRNA-AcrIIA4 ternary complex

5VW1 の概要

• エントリーDOI: 10.2210/pdb5vw1/pdb
• 分子名称: CRISPR-associated endonuclease Cas9/Csn1, sgRNA, anti-CRISPR protein AcrIIA4, ... (8 entities in total)
• 機能のキーワード: type ii crispr-cas endonculease: cas9: structure: endonuclease: anti-crispr protein: inhibition of cas9: ruvc catalytic pocket: sequence-specific pam recognition: genome editing tool, hydrolase
• 由来する生物種: Streptococcus pyogenes serotype M1; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 197146.49

構造登録者

Yang, H.,Patel, D.J. (登録日: 2017-05-21, 公開日: 2017-06-28, 最終更新日: 2023-10-04)

• 主引用文献: Yang, H.,Patel, D.J.; Inhibition Mechanism of an Anti-CRISPR Suppressor AcrIIA4 Targeting SpyCas9.; Mol. Cell, 67:117-127.e5, 2017; PubMed: 28602637; DOI: 10.1016/j.molcel.2017.05.024

実験手法

X-RAY DIFFRACTION (2.598 Å)