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5VO5

Crystal structure of Lgd-Shrub complex, single chain fusion

Summary for 5VO5
Entry DOI10.2210/pdb5vo5/pdb
DescriptorCoiled-coil and C2 domain-containing protein 1-like,GH13992p (2 entities in total)
Functional Keywordsescrt, transport protein
Biological sourceDrosophila melanogaster (Fruit fly)
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Total number of polymer chains1
Total formula weight20906.72
Authors
McMillan, B.J.,Seegar, T.C.M.,Blacklow, S.C. (deposition date: 2017-05-02, release date: 2017-06-14, Last modification date: 2023-10-04)
Primary citationMcMillan, B.J.,Tibbe, C.,Drabek, A.A.,Seegar, T.C.M.,Blacklow, S.C.,Klein, T.
Structural Basis for Regulation of ESCRT-III Complexes by Lgd.
Cell Rep, 19:1750-1757, 2017
Cited by
PubMed Abstract: The ESCRT-III complex induces outward membrane budding and fission through homotypic polymerization of its core component Shrub/CHMP4B. Shrub activity is regulated by its direct interaction with a protein called Lgd in flies, or CC2D1A or B in humans. Here, we report the structural basis for this interaction and propose a mechanism for regulation of polymer assembly. The isolated third DM14 repeat of Lgd binds Shrub, and an Lgd fragment containing only this DM14 repeat and its C-terminal C2 domain is sufficient for in vivo function. The DM14 domain forms a helical hairpin with a conserved, positively charged tip, that, in the structure of a DM14 domain-Shrub complex, occupies a negatively charged surface of Shrub that is otherwise used for homopolymerization. Lgd mutations at this interface disrupt its function in flies, confirming functional importance. Together, these data argue that Lgd regulates ESCRT activity by controlling access to the Shrub self-assembly surface.
PubMed: 28564595
DOI: 10.1016/j.celrep.2017.05.026
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.004 Å)
Structure validation

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