5VO5
Crystal structure of Lgd-Shrub complex, single chain fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.970, 26.990, 82.840 |
Unit cell angles | 90.00, 125.95, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.970 | 2.076 |
High resolution limit [Å] | 2.004 | 2.004 |
Rmerge | 0.066 | 1.549 |
Rpim | 0.934 | |
Number of reflections | 27084 | 1428 |
<I/σ(I)> | 11.44 | 0.95 |
Completeness [%] | 97.4 | 93.85 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.999 | 0.365 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 18% PEG 3350 0.1 M sodium citrate pH 5.5 Cryopreserved using 20% glycerol, 25% PEG 3350, 0.1 M sodium citrate 5.4 |