5VM5
Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9, with Ser bound
Summary for 5VM5
Entry DOI | 10.2210/pdb5vm5/pdb |
Descriptor | Tryptophan synthase beta chain 1, [3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE, SODIUM ION, ... (5 entities in total) |
Functional Keywords | plp, non-canonical amino acid, directed evolution, allostery, lyase |
Biological source | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
Total number of polymer chains | 4 |
Total formula weight | 175048.25 |
Authors | Buller, A.R.,van Roye, P. (deposition date: 2017-04-26, release date: 2017-05-24, Last modification date: 2024-03-13) |
Primary citation | Buller, A.R.,van Roye, P.,Cahn, J.K.B.,Scheele, R.A.,Herger, M.,Arnold, F.H. Directed Evolution Mimics Allosteric Activation by Stepwise Tuning of the Conformational Ensemble. J. Am. Chem. Soc., 140:7256-7266, 2018 Cited by PubMed: 29712420DOI: 10.1021/jacs.8b03490 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.67 Å) |
Structure validation
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