5VM5

Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB2B9, with Ser bound

Summary for 5VM5

Entry DOI	10.2210/pdb5vm5/pdb
Descriptor	Tryptophan synthase beta chain 1, [3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-SERINE, SODIUM ION, ... (5 entities in total)
Functional Keywords	plp, non-canonical amino acid, directed evolution, allostery, lyase
Biological source	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Total number of polymer chains	4
Total formula weight	175048.25
Authors	Buller, A.R.,van Roye, P. (deposition date: 2017-04-26, release date: 2017-05-24, Last modification date: 2024-03-13)
Primary citation	Buller, A.R.,van Roye, P.,Cahn, J.K.B.,Scheele, R.A.,Herger, M.,Arnold, F.H. Directed Evolution Mimics Allosteric Activation by Stepwise Tuning of the Conformational Ensemble. J. Am. Chem. Soc., 140:7256-7266, 2018 Cited by PubMed: 29712420 DOI: 10.1021/jacs.8b03490 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.67 Å)

Structure validation