5VI7

Crystal structure of the Zika virus NS3 helicase

Summary for 5VI7

• Entry DOI: 10.2210/pdb5vi7/pdb
• Descriptor: helicase, SULFATE ION (3 entities in total)
• Functional Keywords: helicase, zika virus, viral protein
• Biological source: Zika virus (ZIKV)
• Cellular location: Capsid protein C: Virion . Peptide pr: Secreted . Small envelope protein M: Virion membrane ; Multi-pass membrane protein . Envelope protein E: Virion membrane ; Multi-pass membrane protein . Non-structural protein 1: Secreted . Non-structural protein 2A: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Multi-pass membrane protein . Serine protease NS3: Host endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Non-structural protein 4A: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . Non-structural protein 4B: Host endoplasmic reticulum membrane ; Multi-pass membrane protein . RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side: Q32ZE1
• Total number of polymer chains: 1
• Total formula weight: 50366.33

Authors

Bukrejewska, M.,Derewenda, U.,Derewenda, Z.S. (deposition date: 2017-04-14, release date: 2017-09-06, Last modification date: 2023-10-04)

Primary citation

Bukrejewska, M.,Derewenda, U.,Radwanska, M.,Engel, D.A.,Derewenda, Z.S.
Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain.
Acta Crystallogr D Struct Biol, 73:767-774, 2017

PubMed Abstract: Two nonstructural proteins encoded by Zika virus strain MR766 RNA, a methyltransferase and a helicase, were crystallized and their structures were solved and refined at 2.10 and 2.01 Å resolution, respectively. The NS5 methyltransferase contains a bound S-adenosyl-L-methionine (SAM) co-substrate. The NS3 helicase is in the apo form. Comparison with published crystal structures of the helicase in the apo, nucleotide-bound and single-stranded RNA (ssRNA)-bound states suggests that binding of ssRNA to the helicase may occur through conformational selection rather than induced fit.

PubMed: 28876240
DOI: 10.1107/S2059798317010737

Experimental method

X-RAY DIFFRACTION (2.005 Å)