5VI7
Crystal structure of the Zika virus NS3 helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-12-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.606, 79.468, 119.769 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.734 - 2.005 |
R-factor | 0.1671 |
Rwork | 0.165 |
R-free | 0.20710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k8u |
RMSD bond length | 0.003 |
RMSD bond angle | 0.710 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.734 |
High resolution limit [Å] | 2.005 |
Number of reflections | 23840 |
<I/σ(I)> | 2.61 |
Completeness [%] | 83.1 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | PEG 400, cacodylate, lithium sulfate |