5V94

Pekin duck egg lysozyme isoform III (DEL-III), cubic form

Summary for 5V94

• Entry DOI: 10.2210/pdb5v94/pdb
• Related: 5V8G 5V92
• Descriptor: lysozyme isoform III, PHOSPHATE ION, (4R)-2-METHYLPENTANE-2,4-DIOL, ... (5 entities in total)
• Functional Keywords: lysozyme, glycosyl hydrolase, n-acetylmuramidase, hydrolase
• Biological source: Anas platyrhynchos (Mallard, Pekin duck)
• Total number of polymer chains: 2
• Total formula weight: 30162.33

Authors

Langley, D.B.,Christ, D. (deposition date: 2017-03-22, release date: 2017-11-15, Last modification date: 2023-10-04)

Primary citation

Langley, D.B.,Crossett, B.,Schofield, P.,Jackson, J.,Zeraati, M.,Maltby, D.,Christie, M.,Burnett, D.,Brink, R.,Goodnow, C.,Christ, D.
Structural basis of antigen recognition: crystal structure of duck egg lysozyme.
Acta Crystallogr D Struct Biol, 73:910-920, 2017

PubMed Abstract: Duck egg lysozyme (DEL) is a widely used model antigen owing to its capacity to bind with differential affinity to anti-chicken egg lysozyme antibodies. However, no structures of DEL have so far been reported, and the situation had been complicated by the presence of multiple isoforms and conflicting reports of primary sequence. Here, the structures of two DEL isoforms from the eggs of the commonly used Pekin duck (Anas platyrhynchos) are reported. Using structural analyses in combination with mass spectrometry, non-ambiguous DEL primary sequences are reported. Furthermore, the structures and sequences determined here enable rationalization of the binding affinity of DEL for well documented landmark anti-lysozyme antibodies.

PubMed: 29095163
DOI: 10.1107/S2059798317013730

Experimental method

X-RAY DIFFRACTION (1.65 Å)