5V8G
Pekin duck lysozyme isoform I (DEL-I)
Summary for 5V8G
| Entry DOI | 10.2210/pdb5v8g/pdb |
| Related | 5V92 5V94 |
| Descriptor | lysozyme isoform I, THIOCYANATE ION, CHLORIDE ION, ... (4 entities in total) |
| Functional Keywords | lysozyme, hydrolase, n-acetylmuramidase |
| Biological source | Anas platyrhynchos (Mallard, Pekin duck) |
| Total number of polymer chains | 1 |
| Total formula weight | 14251.48 |
| Authors | Langley, D.B.,Christ, D. (deposition date: 2017-03-22, release date: 2017-11-15, Last modification date: 2024-11-20) |
| Primary citation | Langley, D.B.,Crossett, B.,Schofield, P.,Jackson, J.,Zeraati, M.,Maltby, D.,Christie, M.,Burnett, D.,Brink, R.,Goodnow, C.,Christ, D. Structural basis of antigen recognition: crystal structure of duck egg lysozyme. Acta Crystallogr D Struct Biol, 73:910-920, 2017 Cited by PubMed Abstract: Duck egg lysozyme (DEL) is a widely used model antigen owing to its capacity to bind with differential affinity to anti-chicken egg lysozyme antibodies. However, no structures of DEL have so far been reported, and the situation had been complicated by the presence of multiple isoforms and conflicting reports of primary sequence. Here, the structures of two DEL isoforms from the eggs of the commonly used Pekin duck (Anas platyrhynchos) are reported. Using structural analyses in combination with mass spectrometry, non-ambiguous DEL primary sequences are reported. Furthermore, the structures and sequences determined here enable rationalization of the binding affinity of DEL for well documented landmark anti-lysozyme antibodies. PubMed: 29095163DOI: 10.1107/S2059798317013730 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.2 Å) |
Structure validation
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