5UNG

XFEL structure of human angiotensin II type 2 receptor (Orthorhombic form) in complex with compound 1 (N-benzyl-N-(2-ethyl-4-oxo-3-{[2'-(2H-tetrazol-5-yl)[1,1'-biphenyl]-4-yl] methyl}-3,4-dihydroquinazolin-6-yl)thiophene-2-carboxamide)

Summary for 5UNG

• Entry DOI: 10.2210/pdb5ung/pdb
• Related: 5UNF 5UNH
• Descriptor: Chimera protein of Type-2 angiotensin II receptor and Soluble cytochrome b562, N-benzyl-N-(2-ethyl-4-oxo-3-{[2'-(2H-tetrazol-5-yl)[1,1'-biphenyl]-4-yl]methyl}-3,4-dihydroquinazolin-6-yl)thiophene-2-carboxamide, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (5 entities in total)
• Functional Keywords: human angiotensin ii receptor complex, gpcr signaling, gpcr, bril, membrane protein, lcp, xfel, blood pressure regulation, orthorhombic crystal, compound 1 (cpd 1), signaling protein
• Biological source: Escherichia coli; More
• Total number of polymer chains: 1
• Total formula weight: 47769.26

Authors

Zhang, H.,Han, G.W.,Batyuk, A.,Ishchenko, A.,White, K.L.,Patel, N.,Sadybekov, A.,Zamlynny, B.,Rudd, M.T.,Hollenstein, K.,Tolstikova, A.,White, T.A.,Hunter, M.S.,Weierstall, U.,Liu, W.,Babaoglu, K.,Moore, E.L.,Katz, R.D.,Shipman, J.M.,Garcia-Calvo, M.,Sharma, S.,Sheth, P.,Soisson, S.M.,Stevens, R.C.,Katritch, V.,Cherezov, V. (deposition date: 2017-01-30, release date: 2017-04-05, Last modification date: 2023-10-04)

Primary citation

Zhang, H.,Han, G.W.,Batyuk, A.,Ishchenko, A.,White, K.L.,Patel, N.,Sadybekov, A.,Zamlynny, B.,Rudd, M.T.,Hollenstein, K.,Tolstikova, A.,White, T.A.,Hunter, M.S.,Weierstall, U.,Liu, W.,Babaoglu, K.,Moore, E.L.,Katz, R.D.,Shipman, J.M.,Garcia-Calvo, M.,Sharma, S.,Sheth, P.,Soisson, S.M.,Stevens, R.C.,Katritch, V.,Cherezov, V.
Structural basis for selectivity and diversity in angiotensin II receptors.
Nature, 544:327-332, 2017

PubMed Abstract: The angiotensin II receptors ATR and ATR serve as key components of the renin-angiotensin-aldosterone system. ATR has a central role in the regulation of blood pressure, but the function of ATR is unclear and it has a variety of reported effects. To identify the mechanisms that underlie the differences in function and ligand selectivity between these receptors, here we report crystal structures of human ATR bound to an ATR-selective ligand and to an ATR/ATR dual ligand, capturing the receptor in an active-like conformation. Unexpectedly, helix VIII was found in a non-canonical position, stabilizing the active-like state, but at the same time preventing the recruitment of G proteins or β-arrestins, in agreement with the lack of signalling responses in standard cellular assays. Structure-activity relationship, docking and mutagenesis studies revealed the crucial interactions for ligand binding and selectivity. Our results thus provide insights into the structural basis of the distinct functions of the angiotensin receptors, and may guide the design of new selective ligands.

PubMed: 28379944
DOI: 10.1038/nature22035

Experimental method

X-RAY DIFFRACTION (2.8 Å)