5UC0

Crystal Structure of Beta-barrel-like, Uncharacterized Protein of COG5400 from Brucella abortus

Summary for 5UC0

• Entry DOI: 10.2210/pdb5uc0/pdb
• Descriptor: Uncharacterized Protein COG5400, SULFATE ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: beta barrel-like, beta-structure, chicago center for functional annotation, midwest center for structural genomics, mcsg, psi-biology, hydrolase
• Biological source: Brucella abortus (strain 2308)
• Total number of polymer chains: 2
• Total formula weight: 35705.94

Authors

Kim, Y.,Bigelow, L.,Endres, M.,Babnigg, G.,Crosson, S.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (deposition date: 2016-12-21, release date: 2017-02-08, Last modification date: 2020-01-01)

Primary citation

Herrou, J.,Willett, J.W.,Fiebig, A.,Varesio, L.M.,Czyz, D.M.,Cheng, J.X.,Ultee, E.,Briegel, A.,Bigelow, L.,Babnigg, G.,Kim, Y.,Crosson, S.
Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus.
Mol. Microbiol., 2018

PubMed Abstract: Molecular components of the Brucella abortus cell envelope play a major role in its ability to infect, colonize and survive inside mammalian host cells. In this study, we have defined a role for a conserved gene of unknown function in B. abortus envelope stress resistance and infection. Expression of this gene, which we name eipA, is directly activated by the essential cell cycle regulator, CtrA. eipA encodes a soluble periplasmic protein that adopts an unusual eight-stranded β-barrel fold. Deletion of eipA attenuates replication and survival in macrophage and mouse infection models, and results in sensitivity to treatments that compromise the cell envelope integrity. Transposon disruption of genes required for LPS O-polysaccharide biosynthesis is synthetically lethal with eipA deletion. This genetic connection between O-polysaccharide and eipA is corroborated by our discovery that eipA is essential in Brucella ovis, a naturally rough species that harbors mutations in several genes required for O-polysaccharide production. Conditional depletion of eipA expression in B. ovis results in a cell chaining phenotype, providing evidence that eipA directly or indirectly influences cell division in Brucella. We conclude that EipA is a molecular determinant of Brucella virulence that functions to maintain cell envelope integrity and influences cell division.

PubMed: 30536925
DOI: 10.1111/mmi.14178

Experimental method

X-RAY DIFFRACTION (1.73 Å)