5UC0
Crystal Structure of Beta-barrel-like, Uncharacterized Protein of COG5400 from Brucella abortus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 63 |
Unit cell lengths | 113.075, 113.075, 64.308 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.642 - 1.730 |
R-factor | 0.179 |
Rwork | 0.176 |
R-free | 0.20760 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((dev_2411: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.089 | 0.761 |
Number of reflections | 49076 | |
<I/σ(I)> | 24.9 | 2.2 |
Completeness [%] | 99.7 | 99.1 |
Redundancy | 7.4 | 7.3 |
CC(1/2) | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate pH 6.5, 30 %(w/v) PEG 8000 |