5U84

Acid ceramidase (ASAH1, aCDase) from common minke whale, Cys143Ala, uncleaved

Summary for 5U84

• Entry DOI: 10.2210/pdb5u84/pdb
• Related: 5U7Z 5U81
• Descriptor: Acid ceramidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: ceramidase, ceramide, ntn-hydrolase, hydrolase
• Biological source: Balaenoptera acutorostrata (minke whale)
• Total number of polymer chains: 2
• Total formula weight: 98634.37

Authors

Gebai, A.,Gorelik, A.,Illes, K.,Nagar, B. (deposition date: 2016-12-13, release date: 2018-03-28, Last modification date: 2024-10-09)

Primary citation

Gebai, A.,Gorelik, A.,Li, Z.,Illes, K.,Nagar, B.
Structural basis for the activation of acid ceramidase.
Nat Commun, 9:1621-1621, 2018

PubMed Abstract: Acid ceramidase (aCDase, ASAH1) hydrolyzes lysosomal membrane ceramide into sphingosine, the backbone of all sphingolipids, to regulate many cellular processes. Abnormal function of aCDase leads to Farber disease, spinal muscular atrophy with progressive myoclonic epilepsy, and is associated with Alzheimer's, diabetes, and cancer. Here, we present crystal structures of mammalian aCDases in both proenzyme and autocleaved forms. In the proenzyme, the catalytic center is buried and protected from solvent. Autocleavage triggers a conformational change exposing a hydrophobic channel leading to the active site. Substrate modeling suggests distinct catalytic mechanisms for substrate hydrolysis versus autocleavage. A hydrophobic surface surrounding the substrate binding channel appears to be a site of membrane attachment where the enzyme accepts substrates facilitated by the accessory protein, saposin-D. Structural mapping of disease mutations reveals that most would destabilize the protein fold. These results will inform the rational design of aCDase inhibitors and recombinant aCDase for disease therapeutics.

PubMed: 29692406
DOI: 10.1038/s41467-018-03844-2

Experimental method

X-RAY DIFFRACTION (2.34 Å)