5U5Q

12 Subunit RNA Polymerase II at Room Temperature collected using SFX

Summary for 5U5Q

• Entry DOI: 10.2210/pdb5u5q/pdb
• Descriptor: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (14 entities in total)
• Functional Keywords: dna-directed rna polymerase, transcription, transferase, serial femtosecond crystallography
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 12
• Total formula weight: 514705.80

Authors

Bushnell, D.A.,Oberthur, D.,Mariani, V.,Yefanov, O.,Tolstikova, A.,Barty, A. (deposition date: 2016-12-07, release date: 2017-03-29, Last modification date: 2024-11-20)

Primary citation

Oberthuer, D.,Knoska, J.,Wiedorn, M.O.,Beyerlein, K.R.,Bushnell, D.A.,Kovaleva, E.G.,Heymann, M.,Gumprecht, L.,Kirian, R.A.,Barty, A.,Mariani, V.,Tolstikova, A.,Adriano, L.,Awel, S.,Barthelmess, M.,Dorner, K.,Xavier, P.L.,Yefanov, O.,James, D.R.,Nelson, G.,Wang, D.,Calvey, G.,Chen, Y.,Schmidt, A.,Szczepek, M.,Frielingsdorf, S.,Lenz, O.,Snell, E.,Robinson, P.J.,Sarler, B.,Belsak, G.,Macek, M.,Wilde, F.,Aquila, A.,Boutet, S.,Liang, M.,Hunter, M.S.,Scheerer, P.,Lipscomb, J.D.,Weierstall, U.,Kornberg, R.D.,Spence, J.C.,Pollack, L.,Chapman, H.N.,Bajt, S.
Double-flow focused liquid injector for efficient serial femtosecond crystallography.
Sci Rep, 7:44628-44628, 2017

PubMed Abstract: Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double-flow focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices [corrected].

PubMed: 28300169
DOI: 10.1038/srep44628

Experimental method

X-RAY DIFFRACTION (3.8 Å)