5U5Q

12 Subunit RNA Polymerase II at Room Temperature collected using SFX

Summary for 5U5Q

DescriptorDNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, ... (14 entities in total)
Functional Keywordsdna-directed rna polymerase, transcription, transferase, serial femtosecond crystallography
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Total number of polymer chains12
Total molecular weight514705.8
Authors
Bushnell, D.A.,Oberthur, D.,Mariani, V.,Yefanov, O.,Tolstikova, A.,Barty, A. (deposition date: 2016-12-07, release date: 2017-03-29, Last modification date: 2018-09-19)
Primary citation
Oberthuer, D.,Knoska, J.,Wiedorn, M.O.,Beyerlein, K.R.,Bushnell, D.A.,Kovaleva, E.G.,Heymann, M.,Gumprecht, L.,Kirian, R.A.,Barty, A.,Mariani, V.,Tolstikova, A.,Adriano, L.,Awel, S.,Barthelmess, M.,Dorner, K.,Xavier, P.L.,Yefanov, O.,James, D.R.,Nelson, G.,Wang, D.,Calvey, G.,Chen, Y.,Schmidt, A.,Szczepek, M.,Frielingsdorf, S.,Lenz, O.,Snell, E.,Robinson, P.J.,Sarler, B.,Belsak, G.,Macek, M.,Wilde, F.,Aquila, A.,Boutet, S.,Liang, M.,Hunter, M.S.,Scheerer, P.,Lipscomb, J.D.,Weierstall, U.,Kornberg, R.D.,Spence, J.C.,Pollack, L.,Chapman, H.N.,Bajt, S.
Double-flow focused liquid injector for efficient serial femtosecond crystallography.
Sci Rep, 7:44628-44628, 2017
PubMed: 28300169 (PDB entries with the same primary citation)
DOI: 10.1038/srep44628
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.8 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.271133.6%3.6%0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5u5q
no rotation
Molmil generated image of 5u5q
rotated about x axis by 90°
Molmil generated image of 5u5q
rotated about y axis by 90°