5U5Q
12 Subunit RNA Polymerase II at Room Temperature collected using SFX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 293.15 |
Detector technology | PIXEL |
Collection date | 2015-05-26 |
Detector | CS-PAD CXI-2 |
Wavelength(s) | 1.55 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 223.400, 396.800, 287.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.611 - 3.800 |
R-factor | 0.219 |
Rwork | 0.218 |
R-free | 0.27060 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.616 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Refinement software | PHENIX ((dev_2356: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.611 |
High resolution limit [Å] | 3.800 |
Number of reflections | 123899 |
<I/σ(I)> | 2.41 |
Completeness [%] | 99.0 |
Redundancy | 54.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.3 | 289.65 | 21-22% PEG 400, 5% PEG 5%, 100 mM Ammonium Phosphate pH 6.3, 100 mM NaCl, 1 mM ZnCl2 and 10 mM DTT |