5U4Y

IgG Fc bound to 3 helix of the B-domain from Protein A

5U4Y の概要

• エントリーDOI: 10.2210/pdb5u4y/pdb
• 関連するPDBエントリー: 1L6X 5U52 5U66
• 分子名称: IgG1 fc, Immunoglobulin G-binding protein A, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: igg1, fc, helix, b-domain, protein a, transcription
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 63308.70

構造登録者

Ultsch, M.H.,Eigenbrot, C. (登録日: 2016-12-06, 公開日: 2017-05-17, 最終更新日: 2024-11-20)

主引用文献

Ultsch, M.,Braisted, A.,Maun, H.R.,Eigenbrot, C.
3-2-1: Structural insights from stepwise shrinkage of a three-helix Fc-binding domain to a single helix.
Protein Eng. Des. Sel., 30:619-625, 2017

PubMed Abstract: The well-studied B-domain from Staphylococcal protein A is a 59 amino acid three-helix bundle that binds the Fc portion of IgG with a dissociation constant of ~35 nM. The B-domain variant bearing a Gly to Ala mutation (=Z-domain) has been the subject of efforts to minimize a domain's size while retaining its function. We report X-ray crystallographic characterization of three steps in such a process using complexes with Fc: the full three-helix Z-domain, a 34 amino acid two-helix version called Z34C and a 13 amino acid single helix stabilized with an exo-helix tether, called LH1.

PubMed: 28475752
DOI: 10.1093/protein/gzx029

実験手法

X-RAY DIFFRACTION (2.4994 Å)