5U4Y
IgG Fc bound to 3 helix of the B-domain from Protein A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 273 |
Detector technology | CCD |
Collection date | 1996-12-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 105.950, 152.260, 46.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.075 - 2.499 |
R-factor | 0.1949 |
Rwork | 0.193 |
R-free | 0.23010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fc1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.962 |
Data reduction software | HKL-2000 (1.3.0) |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.590 |
High resolution limit [Å] | 2.499 | 2.499 |
Rmerge | 0.285 | |
Number of reflections | 25214 | |
<I/σ(I)> | 30.4 | 3.9 |
Completeness [%] | 97.1 | 99.1 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG 3400, 0.1M magnesium acetate, 0.1 M HEPES pH 7.0. Protein concentration 5mg/mL. |