5TZS

Architecture of the yeast small subunit processome

Summary for 5TZS

• Entry DOI: 10.2210/pdb5tzs/pdb
• EMDB information: 8473
• Descriptor: 5' external transcribed spacer, 5' domain-associated, 3' domain-associated, ... (53 entities in total)
• Functional Keywords: ribosome assembly, translation
• Biological source: Saccharomyces cerevisiae S288c (yeast); More
• Total number of polymer chains: 58
• Total formula weight: 2258551.34

Authors

Chaker-Margot, M.,Barandun, J.,Hunziker, M.,Klinge, S. (deposition date: 2016-11-22, release date: 2016-12-21, Last modification date: 2024-03-13)

Primary citation

Chaker-Margot, M.,Barandun, J.,Hunziker, M.,Klinge, S.
Architecture of the yeast small subunit processome.
Science, 355:-, 2017

PubMed Abstract: The small subunit (SSU) processome, a large ribonucleoprotein particle, organizes the assembly of the eukaryotic small ribosomal subunit by coordinating the folding, cleavage, and modification of nascent pre-ribosomal RNA (rRNA). Here, we present the cryo-electron microscopy structure of the yeast SSU processome at 5.1-angstrom resolution. The structure reveals how large ribosome biogenesis complexes assist the 5' external transcribed spacer and U3 small nucleolar RNA in providing an intertwined RNA-protein assembly platform for the separate maturation of 18S rRNA domains. The strategic placement of a molecular motor at the center of the particle further suggests a mechanism for mediating conformational changes within this giant particle. This study provides a structural framework for a mechanistic understanding of eukaryotic ribosome assembly in the model organism Saccharomyces cerevisiae.

PubMed: 27980088
DOI: 10.1126/science.aal1880

Experimental method

ELECTRON MICROSCOPY (5.1 Å)