Cryo-electron microscopy structure of a bovine CLC-K chloride channel, main (class 1) conformation

> Summary

Summary for 5TQQ

EMDB informationEMD-8435 EMD-8454
DescriptorChloride channel protein, Monoclonal antibody, Fab fragment, light chain, Monoclonal antibody, Fab fragment, heavy chain
Functional Keywordsclc, chloride channel, membrane, kidney, transport protein
Biological sourceBos taurus (Bovine)
Cellular locationMembrane ; Multi-pass membrane protein  E1B792
Total number of polymer chains6
Total molecular weight195957.31
Park, E.,MacKinnon, R. (deposition date: 2016-10-24, release date: 2017-01-04, Last modification date: 2017-02-08)
Primary citation
Park, E.,Campbell, E.B.,MacKinnon, R.
Structure of a CLC chloride ion channel by cryo-electron microscopy.
Nature, 541:500-505, 2017
PubMed: 28002411
DOI: 10.1038/nature20812
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliers60.1%0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5tqq
no rotation
Molmil generated image of 5tqq
rotated about x axis by 90°
Molmil generated image of 5tqq
rotated about y axis by 90°

> Structural details

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains6
Total molecular weight195957.3
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight195957.3
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:ELECTRON MICROSCOPY

Cell axes312.000312.000312.000
Cell angles90.0090.0090.00
SpacegroupP 1
Resolution limits -
the highest resolution shell value -

Data Collection Statistics

Resolution limits -
the highest resolution shell value -

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details


Functional Information from GO Data

A0016021cellular_componentintegral component of membrane
A0005247molecular_functionvoltage-gated chloride channel activity
A1902476biological_processchloride transmembrane transport
B0016021cellular_componentintegral component of membrane
B0005247molecular_functionvoltage-gated chloride channel activity
B1902476biological_processchloride transmembrane transport

Functional Information from PDB Data

site_idNumber of ResiduesDetails

Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails

Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails

Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails

Catalytic Information from CSA

site_idNumber of ResiduesDetails

> Sequence Neighbor

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