5TD8

Crystal structure of an Extended Dwarf Ndc80 Complex

Summary for 5TD8

• Entry DOI: 10.2210/pdb5td8/pdb
• Descriptor: Kinetochore protein NDC80, Kinetochore protein NUF2, Kinetochore protein SPC24, ... (6 entities in total)
• Functional Keywords: rwd, ch, coiled-coil, tetramer, ndc80, kinetochore, replication, nanobody
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 5
• Total formula weight: 101639.06

Authors

Valverde, R.,Ingram, J.,Harrison, S.C. (deposition date: 2016-09-17, release date: 2016-11-16, Last modification date: 2024-10-16)

Primary citation

Valverde, R.,Ingram, J.,Harrison, S.C.
Conserved Tetramer Junction in the Kinetochore Ndc80 Complex.
Cell Rep, 17:1915-1922, 2016

PubMed Abstract: The heterotetrameric Ndc80 complex establishes connectivity along the principal longitudinal axis of a kinetochore. Its two heterodimeric subcomplexes, each with a globular end and a coiled-coil shaft, connect end-to-end to create a ∼600 Å long rod spanning the gap from centromere-proximal structures to spindle microtubules. Neither subcomplex has a known function on its own, but the heterotetrameric organization and the characteristics of the junction are conserved from yeast to man. We have determined crystal structures of two shortened ("dwarf") Ndc80 complexes that contain the full tetramer junction and both globular ends. The junction connects two α-helical coiled coils through regions of four-chain and three-chain overlap. The complexity of its structure depends on interactions among conserved amino-acid residues, suggesting a binding site for additional cellular factor(s) not yet identified.

PubMed: 27851957
DOI: 10.1016/j.celrep.2016.10.065

Experimental method

X-RAY DIFFRACTION (7.531 Å)