5TD8

Crystal structure of an Extended Dwarf Ndc80 Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Kinetochore protein NDC80	polymer	279	33256.9	1	UniProt (P40460) Pfam (PF03801) Pfam (PF18077)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	80 kDa spindle component protein,Nuclear division cycle protein 80,Two-hybrid interaction with DMC1 protein 3,80 kDa spindle component protein,Nuclear division cycle protein 80,Two-hybrid interaction with DMC1 protein 3
2	B (B)	Kinetochore protein NUF2	polymer	198	23246.3	1	UniProt (P33895) Pfam (PF03800)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
3	C (C)	Kinetochore protein SPC24	polymer	114	13469.3	1	UniProt (Q04477) Pfam (PF08286)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	D (D)	Kinetochore protein SPC25	polymer	129	14568.6	1	UniProt (P40014)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
5	E (E)	nanobody	polymer	145	15894.4	1		Vicugna pacos
6	F, G, H, I, J... (A, B, D)	MERCURY (II) ION	non-polymer		200.6	6	Chemie (HG)

Sequence modifications

A: 114 - 318 (UniProt: P40460)

PDB	External Database	Details
Ser 111	-	expression tag
Asn 112	-	expression tag
Ala 113	-	expression tag

A: 621 - 691 (UniProt: P40460)
B: 1 - 153 (UniProt: P33895)
B: 407 - 451 (UniProt: P33895)
C: 1 - 62 (UniProt: Q04477)
C: 162 - 213 (UniProt: Q04477)
D: 1 - 45 (UniProt: P40014)
D: 138 - 221 (UniProt: P40014)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	100435.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1203.5
All*	Total formula weight	101639.1

*Water molecules are not included.