5T2O
Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP011377 gene; harbors 53 point mutations relative to wild-type I-OnuI
Summary for 5T2O
Entry DOI | 10.2210/pdb5t2o/pdb |
Related | 5T2H 5T2J 5T2N |
Descriptor | I-OnuI_e-ag011377, DNA (26-MER), CALCIUM ION, ... (5 entities in total) |
Functional Keywords | meganuclease, engineered protein, dna complex, homing endonuclease, hydrolase-dna complex, hydrolase/dna |
Biological source | synthetic construct More |
Total number of polymer chains | 3 |
Total formula weight | 50948.86 |
Authors | Stoddard, B.L.,Werther, R. (deposition date: 2016-08-23, release date: 2017-05-03, Last modification date: 2023-10-04) |
Primary citation | Werther, R.,Hallinan, J.P.,Lambert, A.R.,Havens, K.,Pogson, M.,Jarjour, J.,Galizi, R.,Windbichler, N.,Crisanti, A.,Nolan, T.,Stoddard, B.L. Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity. Nucleic Acids Res., 45:8621-8634, 2017 Cited by PubMed: 28637173DOI: 10.1093/nar/gkx544 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.801 Å) |
Structure validation
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