5T0A

Crystal Structure of Heparan Sulfate 6-O-Sulfotransferase with bound PAP and heptasaccharide substrate

5T0A の概要

• エントリーDOI: 10.2210/pdb5t0a/pdb
• 関連するPDBエントリー: 5T03 5T05
• 関連するBIRD辞書のPRD_ID: PRD_900010
• 分子名称: maltose binding protein - heparan sulfate 6-O-sulfotransferase isoform 3 fusion protein, CHLORIDE ION, 2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid, ... (11 entities in total)
• 機能のキーワード: heparan sulfate, sulfotransferase, complex, transferase
• 由来する生物種: Escherichia coli O157:H7; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 164082.10

構造登録者

Pedersen, L.C.,Moon, A.F.,Krahn, J.M.,Liu, J. (登録日: 2016-08-15, 公開日: 2017-02-01, 最終更新日: 2024-11-06)

主引用文献

Xu, Y.,Moon, A.F.,Xu, S.,Krahn, J.M.,Liu, J.,Pedersen, L.C.
Structure Based Substrate Specificity Analysis of Heparan Sulfate 6-O-Sulfotransferases.
ACS Chem. Biol., 12:73-82, 2017

PubMed Abstract: Heparan sulfate (HS) is a sulfated polysaccharide exhibiting essential physiological functions. HS 6-O-sulfotransferase (6-OST) transfers a sulfo group to the 6-OH position of glucosamine units to confer a variety of HS biological activities. There are three different isoforms of 6-OST in the human genome. Here, we report crystal structures of the ternary complex of 6-OST with the sulfo donor analog 3'-phosphoadenosine 5'-phosphate and three different oligosaccharide substrates at 1.95 to 2.1 Å resolutions. Structural and mutational analyses reveal amino acid residues that contribute to catalysis and substrate recognition of 6-OST. Unexpectedly, the structures reveal 6-OST engages HS in a completely different orientation than other HS sulfotransferases and sheds light on the basic HS requirements for specificity. These findings also contribute structural information to understand mutations in human 6-OST isoform 1 associated with the human genetic disease idiopathic hypogonadotropic hypogonadism characterized by incomplete or lack of puberty.

PubMed: 28103688
DOI: 10.1021/acschembio.6b00841

実験手法

X-RAY DIFFRACTION (1.95 Å)