5SVA

Mediator-RNA Polymerase II Pre-Initiation Complex

5SVA の概要

• エントリーDOI: 10.2210/pdb5sva/pdb
• EMDBエントリー: 8305 8307 8308
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (42 entities in total)
• 機能のキーワード: transcriptional initiation, mediator, pre-initiation complex, carboxy-terminal domain (ctd), transcription, transferase-dna complex, transferase/dna
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 40
• 化学式量合計: 1522751.47

構造登録者

Robinson, P.J.,Bushnell, D.A.,Kornberg, R.D. (登録日: 2016-08-05, 公開日: 2016-09-28, 最終更新日: 2024-11-20)

主引用文献

Robinson, P.J.,Trnka, M.J.,Bushnell, D.A.,Davis, R.E.,Mattei, P.J.,Burlingame, A.L.,Kornberg, R.D.
Structure of a Complete Mediator-RNA Polymerase II Pre-Initiation Complex.
Cell, 166:1411-1422.e16, 2016

PubMed Abstract: A complete, 52-protein, 2.5 million dalton, Mediator-RNA polymerase II pre-initiation complex (Med-PIC) was assembled and analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry. The resulting complete Med-PIC structure reveals two components of functional significance, absent from previous structures, a protein kinase complex and the Mediator-activator interaction region. It thereby shows how the kinase and its target, the C-terminal domain of the polymerase, control Med-PIC interaction and transcription.

PubMed: 27610567
DOI: 10.1016/j.cell.2016.08.050

実験手法

ELECTRON MICROSCOPY (15.3 Å)