5OY7

Structure of the 4_601_157 tetranucleosome (P1 form)

5OY7 の概要

• エントリーDOI: 10.2210/pdb5oy7/pdb
• 関連するPDBエントリー: 5OXV
• 分子名称: Histone H2B 1.1, Histone H3, Histone H4, ... (7 entities in total)
• 機能のキーワード: nucleosome, histone, dna, tetranucleosome, chromatin fiber, gene regulation
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 34
• 化学式量合計: 827371.85

構造登録者

Ekundayo, B.,Richmond, T.J.,Schalch, T. (登録日: 2017-09-07, 公開日: 2017-09-20, 最終更新日: 2024-01-17)

主引用文献

Ekundayo, B.,Richmond, T.J.,Schalch, T.
Capturing Structural Heterogeneity in Chromatin Fibers.
J. Mol. Biol., 429:3031-3042, 2017

PubMed Abstract: Chromatin fiber organization is implicated in processes such as transcription, DNA repair and chromosome segregation, but how nucleosomes interact to form higher-order structure remains poorly understood. We solved two crystal structures of tetranucleosomes with approximately 11-bp DNA linker length at 5.8 and 6.7 Å resolution. Minimal intramolecular nucleosome-nucleosome interactions result in a fiber model resembling a flat ribbon that is compatible with a two-start helical architecture, and that exposes histone and DNA surfaces to the environment. The differences in the two structures combined with electron microscopy reveal heterogeneous structural states, and we used site-specific chemical crosslinking to assess the diversity of nucleosome-nucleosome interactions through identification of structure-sensitive crosslink sites that provide a means to characterize fibers in solution. The chromatin fiber architectures observed here provide a basis for understanding heterogeneous chromatin higher-order structures as they occur in a genomic context.

PubMed: 28893533
DOI: 10.1016/j.jmb.2017.09.002

実験手法

X-RAY DIFFRACTION (5.774 Å)