5OOB

COMPLEX OF HUMAN NUCLEAR CAP-BINDING COMPLEX WITH M7GTP AND NELF-E C-TERMINAL PEPTIDE

5OOB の概要

• エントリーDOI: 10.2210/pdb5oob/pdb
• 分子名称: Nuclear cap-binding protein subunit 1, Nuclear cap-binding protein subunit 2, Negative elongation factor E, ... (4 entities in total)
• 機能のキーワード: complex nuclear cap-binding complex m7gtp c-terminal peptide from ars2, nuclear protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus : Q09161 P52298 P18615
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 441432.87

構造登録者

Cusack, S.,Schulze, W.M. (登録日: 2017-08-07, 公開日: 2017-11-15, 最終更新日: 2024-01-17)

主引用文献

Schulze, W.M.,Cusack, S.
Structural basis for mutually exclusive co-transcriptional nuclear cap-binding complexes with either NELF-E or ARS2.
Nat Commun, 8:1302-1302, 2017

PubMed Abstract: Pol II transcribes diverse classes of RNAs that need to be directed into the appropriate nuclear maturation pathway. All nascent Pol II transcripts are 5'-capped and the cap is immediately sequestered by the nuclear cap-binding complex (CBC). Mutually exclusive interactions of CBC with different partner proteins have been implicated in transcript fate determination. Here, we characterise the direct interactions between CBC and NELF-E, a subunit of the negative elongation factor complex, ARS2 and PHAX. Our biochemical and crystal structure results show that the homologous C-terminal peptides of NELF-E and ARS2 bind identically to CBC and in each case the affinity is enhanced when CBC is bound to a cap analogue. Furthermore, whereas PHAX forms a complex with CBC and ARS2, NELF-E binding to CBC is incompatible with PHAX binding. We thus define two mutually exclusive complexes CBC-NELF-E and CBC-ARS2-PHAX, which likely act in respectively earlier and later phases of transcription.

PubMed: 29101316
DOI: 10.1038/s41467-017-01402-w

実験手法

X-RAY DIFFRACTION (2.79 Å)