Crystal structure of haemanthamine bound to the 80S ribosome

This is a large structure.

Summary for 5ON6

Descriptor25S ribosomal RNA, 5S ribosomal RNA, 5.8S ribosomal RNA, ... (88 entities in total)
Functional Keywordsribosome, inhibitor, cancer
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Cellular locationCytoplasm  P0CX45 P14126 P10664 P26321 Q02326 P05737 P17076 P05738 P41805 Q3E757 Q12690 P36105 P05748 P26784 P05740 P0CX49 P0CX82 P0CX23 Q02753 P05749 P0CX41 P04449 P04456 P05743 P0C2H6 P02406 P05747 P14120 P0C2H8 P38061 P05744 P87262 P0CX84 P05745 P49166 P49167 P04650 P0CX87 P0CX27 P0CX25 P39015 P05317 P32905 P33442 P25443 P05750 P0CX35 P26783 P0CX37 P26786 P0CX39 O13516 Q08745 P0CX47 P48589 P05756 P39516 Q01855 P0CX51 P02407 P0CX55 P07280 P38701 P0C0V8 P0C0W1 P0CX29 P0CX31 Q3E792 P39939 P35997 Q3E7X9 P41057 P0CX33 P38011
Ubiquitin: Cytoplasm . 60S ribosomal protein L40-A: Cytoplasm  P0CH08
Ubiquitin: Cytoplasm . 40S ribosomal protein S31: Cytoplasm  P05759 P05759
Total number of polymer chains160
Total molecular weight6484523
Pellegrino, S.,Meyer, M.,Yusupova, G.,Yusupov, M. (deposition date: 2017-08-03, release date: 2018-02-28, Last modification date: 2018-03-14)
Primary citation
Pellegrino, S.,Meyer, M.,Zorbas, C.,Bouchta, S.A.,Saraf, K.,Pelly, S.C.,Yusupova, G.,Evidente, A.,Mathieu, V.,Kornienko, A.,Lafontaine, D.L.J.,Yusupov, M.
The Amaryllidaceae Alkaloid Haemanthamine Binds the Eukaryotic Ribosome to Repress Cancer Cell Growth.
Structure, 26:416-425.e4, 2018
PubMed: 29429877 (PDB entries with the same primary citation)
DOI: 10.1016/j.str.2018.01.009
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.10000240787 Å)

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.252101.0%12.6%7.0%0.52MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5on6
no rotation
Molmil generated image of 5on6
rotated about x axis by 90°
Molmil generated image of 5on6
rotated about y axis by 90°