5ON6

Crystal structure of haemanthamine bound to the 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.148
Spacegroup name	P 1 21 1
Unit cell lengths	303.130, 286.500, 435.660
Unit cell angles	90.00, 98.87, 90.00

Refinement procedure

Resolution	99.836 - 3.100
R-factor	0.222362191325
Rwork	0.222
R-free	0.25201
RMSD bond length	0.006
RMSD bond angle	0.935
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (dev_2450)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.200
High resolution limit [Å]	3.100	99.836	3.100
Rmerge		0.036	2.059
Rmeas	0.304	0.041	2.234
Number of reflections	1317777	36	118838
<I/σ(I)>	9.74	10.31	1.36
Completeness [%]	99.5	75	99
Redundancy	7.48	2.75	6.458
CC(1/2)		0.999	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine